FastQCFastQC Report
Tue 11 Oct 2022
A737_FrCx_S52_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA737_FrCx_S52_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954716
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGC254.6511923E-794.892962
AGACTGC254.6511923E-794.892962
GAGTGGA1000.094.892963
GCCTTGC254.6511923E-794.892962
GGAGCCC353.3287506E-1094.892964
ATCGTGC353.3287506E-1094.892962
GCTCTGC254.6511923E-794.892962
ACAATGC500.094.892962
GCGGGCT353.3287506E-1094.892965
AATCTGC700.094.892962
GGAGTGC254.6511923E-794.892962
TTGCGAC353.3287506E-1094.892964
CGCAGCC254.6511923E-794.892963
GTTTTGC1850.094.892962
TGGATGC254.6511923E-794.892962
GCAATGC500.094.892962
AAGGCCT500.094.892965
GCTCCAC254.6511923E-794.892967
AATGTGC1000.094.892962
ATCCTGC201.7534667E-594.892952
Produced by FastQC (version 0.11.4)