FastQCFastQC Report
Tue 11 Oct 2022
A737_FrCx_S52_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA737_FrCx_S52_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954716
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG14850.1555436381080866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCA353.2923708E-1095.0317151
GTACCGA254.6108835E-795.0317151
ATGATGA700.095.0317151
AGTTTGA450.095.0317151
GGTCGTA353.2923708E-1095.0317151
GGTCGCA156.608804E-495.0317151
GCGGTTA353.2923708E-1095.0317151
TAGGTCA156.608804E-495.0317151
GTAGGTA353.2923708E-1095.0317151
AGGGTCA450.095.0317151
TGCGGTA254.6108835E-795.0317151
AAGCGGA254.6108835E-795.0317151
CCGCTGA156.608804E-495.0317151
CGTTCGA353.2923708E-1095.0317151
GGGACCA156.608804E-495.0317151
GAGGTTA254.6108835E-795.0317151
GATGGTA450.095.0317151
AGTTGTA450.095.0317151
GGAACGA156.608804E-495.0317151
GGTATGA301.2289092E-895.0317151
Produced by FastQC (version 0.11.4)