FastQCFastQC Report
Tue 11 Oct 2022
A737_DRG_S58_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA737_DRG_S58_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221652
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAC950.094.89024
AGGGCCT850.094.89025
ATCCTGC450.094.890192
GATCCGC156.649243E-494.890192
AGGACTG254.65403E-794.890191
CGACACT156.649243E-494.890197
ATCCCGC500.094.890192
ATCGATG254.65403E-794.890191
GAGGCCC4000.094.890194
GGGACAT32850.094.6013266
ACAACGC181750.094.498639
ACACCGC126400.094.47739
GCACAAC103200.094.338527
GGACATG63100.094.288677
GACACCG50900.094.237718
TGGACAT46100.094.169786
ACAGCGC58800.094.1639949
GGACATC31500.094.137097
TGGCCAT24200.094.105986
GGACAGG107750.094.053577
Produced by FastQC (version 0.11.4)