FastQCFastQC Report
Tue 11 Oct 2022
A737_DRG_S58_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA737_DRG_S58_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221652
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG33450.1505636346286457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCGA301.2290911E-895.037581
GTTCGTA600.095.037581
TAGGGCA301.2290911E-895.037581
GTCGGTA600.095.037581
ATGGGCA353.2923708E-1095.0375751
GCGGTTA700.095.0375751
GATGCGA409.094947E-1295.0375751
GCGCGAA254.611229E-795.037571
GTTACGA500.095.037571
GATGGTA1000.095.037571
GCAGGGA254.611229E-795.037571
ATGCGCA254.611229E-795.037571
GCGCTTA450.095.037571
GCTAACC201.7431064E-595.0118948
AGCGTAC353.3105607E-1094.994782
TCGGGAC301.232911E-894.994782
GTAGGAC650.094.994782
TGCGGAC301.232911E-894.994782
TAGTTAT353.3105607E-1094.994782
AGGCAAC353.3105607E-1094.994782
Produced by FastQC (version 0.11.4)