FastQCFastQC Report
Tue 11 Oct 2022
A736_TA_S4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA736_TA_S4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505086
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9010.17838546306965547No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6920.1370063711922326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACC78050.094.887749
GCGAGAA700.094.887733
CCCTGAA201.7531414E-594.887733
AGGTATG353.3287506E-1094.887731
GAAATGA201.7531414E-594.887732
AGCAATG254.6495188E-794.887731
ATGGGAC254.6495188E-794.887733
AGCCGTC254.6495188E-794.887733
AGACTCC2950.094.887736
AACAACG201.7531414E-594.887731
GACAGGA254.6495188E-794.887732
TAGAGAC201.7531414E-594.887733
AGCAAAG254.6495188E-794.887731
AACCGAA201.7531414E-594.887733
TTGGCGA254.6495188E-794.887732
GGCAGAA353.3287506E-1094.887733
GCCTTGA254.6495188E-794.887732
ACAGTGA254.6495188E-794.887732
GCCCAAA254.6495188E-794.887737
GCAAGGA201.7531414E-594.887732
Produced by FastQC (version 0.11.4)