FastQCFastQC Report
Tue 11 Oct 2022
A736_TA_S4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA736_TA_S4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505086
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG7630.1510633832654241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA409.094947E-1295.0540161
TCCGGAA156.6008256E-495.0540161
TCTGCAA156.6008256E-495.0540161
GTGTTGA201.7379663E-595.0540161
TTGATGA254.6012792E-795.0540161
AACGTTA254.6012792E-795.0540161
TGTTTGA500.095.0540161
GTTTGGA254.6012792E-795.0540161
TTTAGGA254.6012792E-795.0540161
TGTTTCA254.6012792E-795.0540161
CGTGGTA156.6008256E-495.0540161
TTAGGTA550.095.0540161
CATGGGA254.6012792E-795.0540161
ATGGGGA156.6008256E-495.0540161
TTAGGGA156.6008256E-495.0540161
CTATGCA254.6012792E-795.0540161
AGCCGTA156.6008256E-495.0540161
CTATGAA254.6012792E-795.0540161
AACCGCA254.6012792E-795.0540161
GCCTTAA156.6008256E-495.0540161
Produced by FastQC (version 0.11.4)