FastQCFastQC Report
Tue 11 Oct 2022
A735_TA_S3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA735_TA_S3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487719
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8090.16587420215328907No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6510.13347849888972954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCC29150.094.8930749
AACAACC700.094.8930749
ATAGGGG353.3287506E-1094.8930741
AGATAGG353.3287506E-1094.8930741
AAGAGAA550.094.8930743
GAGTATC12500.094.8930745
ATGGACT1100.094.8930744
GAATCCA19450.094.8930747
ATATGTC353.3287506E-1094.8930743
AGTATCC8350.094.8930746
ATCAACC77700.094.8930749
AACGGAA353.3287506E-1094.8930743
GGGCGTC353.3287506E-1094.8930743
AGATTGA353.3287506E-1094.8930742
AGACAGA353.3287506E-1094.8930742
ACAGACT550.094.8930744
GACATGT353.3287506E-1094.8930742
CCGCGTA353.3287506E-1094.8930743
CACAATC550.094.8930745
AGAGGAA700.094.8930743
Produced by FastQC (version 0.11.4)