FastQCFastQC Report
Tue 11 Oct 2022
A735_TA_S3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA735_TA_S3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487719
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG6600.13532382375917282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTA409.094947E-1295.047831
TTGATGA254.6028254E-795.047831
GTGTTCA201.7384678E-595.047831
GTTTGGA254.6028254E-795.047831
GTGTTAA409.094947E-1295.047831
TTTAGGA409.094947E-1295.047831
GTTTGCA201.7384678E-595.047831
TTAGGTA353.2923708E-1095.047831
ATGGGGA201.7384678E-595.047831
CGTGGGA201.7384678E-595.047831
TTAGGGA450.095.047831
GGCAGGA201.7384678E-595.047831
GTTCTTA201.7384678E-595.047831
GATGTTA254.6028254E-795.047831
CTACTTA201.7384678E-595.047831
GATGTGA201.7384678E-595.047831
TTGTGTA450.095.047831
GGTGTGA201.7384678E-595.047831
TCCGCAA201.7384678E-595.047831
GGTCGCA201.7384678E-595.047831
Produced by FastQC (version 0.11.4)