FastQCFastQC Report
Tue 11 Oct 2022
A734_TA_S7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA734_TA_S7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934956
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17640.18867198028570328No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14670.15690577952331447No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCGA156.6478894E-494.890692
ACACGGA156.6478894E-494.890692
GAGCTCT156.6478894E-494.890694
CCGATGA156.6478894E-494.890692
CGCGGAC156.6478894E-494.890693
CAGCACA600.094.890695
GGACTAT156.6478894E-494.890694
GATCCCG156.6478894E-494.890691
AGGACTC5500.094.890695
GCTGCGA156.6478894E-494.890692
GCGCCCG156.6478894E-494.890691
GCTGACG156.6478894E-494.890691
GGCCCAT650.094.890696
GGCCCAC650.094.890696
AGTATCC14650.094.890696
GACCAAG156.6478894E-494.890691
GAGAATG156.6478894E-494.890691
CCGAGAC156.6478894E-494.890693
ACAGCCC156.6478894E-494.890694
ACGCTTG156.6478894E-494.890691
Produced by FastQC (version 0.11.4)