FastQCFastQC Report
Tue 11 Oct 2022
A734_Liver_S19_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA734_Liver_S19_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4410607
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78610.1782294364471829No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76940.17444310953118244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGCG353.3287506E-1094.888891
GCATTCG450.094.888891
GCCTGCG156.650045E-494.888891
GCCTGAC600.094.888893
AGCGATG900.094.888891
CATCATG900.094.888891
GCTCCTG156.650045E-494.888891
CATGCGG409.094947E-1294.8888851
GCTGACG254.655176E-794.8888851
AATGCTG1050.094.8888851
GCTTCTG550.094.8888851
AGGCAGG1450.094.8888851
CAGGCGT1450.094.8888852
CGCAATG550.094.8888851
GCATGCG500.094.8888851
CATTTGC201.7545468E-594.8888852
ATCAACC489400.094.829649
ATCCTCC250100.094.792969
AGACTCC29400.094.727516
GAATCCT85700.094.667457
Produced by FastQC (version 0.11.4)