FastQCFastQC Report
Tue 11 Oct 2022
A734_Liver_S19_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA734_Liver_S19_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4410607
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG82920.18800133405674094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC1000.094.99492
TTGCGAT301.2332748E-894.99492
GGTGCAT301.2332748E-894.99492
AAGGGAC1000.094.99492
GGTTCAT650.094.99492
AGGGAAC1000.094.99492
GGGGAAC1150.094.9948962
GTTCTAC1250.094.9948962
AGGGTAC750.094.9948962
GCGTTAC800.094.9948962
ATGGTAT1250.094.9948962
AAGGCAT201.7448472E-594.9948962
TTGGGAC900.094.9948962
TAACAGG4050.094.953985
AGATTGC2000.094.953985
GGTGAGT10450.094.953983
TTCGACT1950.094.953983
AGCAATG353.3105607E-1094.953974
GGAATGG1150.094.953975
AGGTACT2500.094.953973
Produced by FastQC (version 0.11.4)