FastQCFastQC Report
Tue 11 Oct 2022
A734_Heart_S13_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA734_Heart_S13_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651910
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12390.19005690969612368No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8780.1346811676458407No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATC201.7531345E-594.8920067
TGCCCAA750.094.8920066
GCACACC1100.094.8920067
GCGGGCC201.7531345E-594.8920067
GGACATC850.094.8920067
CGACTCT1250.094.8920066
AGGGCCA409.094947E-1294.8920065
GCCAACG409.094947E-1294.8920068
GACTATC201.7531345E-594.8920065
ACACGGC2700.094.8929
GCGACTC2350.094.8925
GGCACTC156.646614E-494.8925
AGGACAC950.094.8926
CGTCTCC4700.094.8926
TGGCACA900.094.8925
GGCCACC950.094.8927
GCACAAC2000.094.8927
CGCTATC353.3287506E-1094.8925
GCTTATC353.3287506E-1094.8925
GTATCTC301.2409146E-894.8927
Produced by FastQC (version 0.11.4)