FastQCFastQC Report
Tue 11 Oct 2022
A734_Heart_S13_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA734_Heart_S13_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651910
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG9970.1529352211194797No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA156.606182E-495.037771
GTGTTTA450.095.037771
GTTTGGA550.095.037771
CTTCGCA156.606182E-495.037771
CGTGGTA301.22781785E-895.037771
TTAGGTA450.095.037771
ATGGGGA301.22781785E-895.037771
GCCTTTA156.606182E-495.037771
TTGGCGA301.22781785E-895.037771
TCGTTCA156.606182E-495.037771
GTACCGA156.606182E-495.037771
GAAGCGA156.606182E-495.037771
GATGTTA700.095.037771
GATGTGA353.2923708E-1095.037771
ATGATGA301.22781785E-895.037771
TCCGCGA156.606182E-495.037771
GGGCTTA156.606182E-495.037771
CACGGTA156.606182E-495.037771
GGTCGCA156.606182E-495.037771
GTTTCGA301.22781785E-895.037771
Produced by FastQC (version 0.11.4)