FastQCFastQC Report
Tue 11 Oct 2022
A734_Gastroc_S10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA734_Gastroc_S10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787613
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14230.18067248763034638No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13070.16594444225780935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC650.094.890699
GAATCCT15700.094.8906867
GCAGACT1050.094.8906864
AGAATCC14150.094.8906866
TGACTCC5000.094.8906866
AGCACAT1000.094.890686
GGAATCC10700.094.890686
CAGCGGA156.647498E-494.890682
AGGTACG156.647498E-494.890681
AGCAACG156.647498E-494.890681
AGCCGGA156.647498E-494.890682
GGCAGGA450.094.890682
TGGAGAC156.647498E-494.890683
GATACGG301.2412784E-894.890681
AGCCGAC201.7534836E-594.890683
AATCATG156.647498E-494.890681
AATCACG201.7534836E-594.890681
GGAGACG156.647498E-494.890681
AGGAGAC301.2412784E-894.890683
GCGCGAG156.647498E-494.890681
Produced by FastQC (version 0.11.4)