FastQCFastQC Report
Tue 11 Oct 2022
A734_Gastroc_S10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA734_Gastroc_S10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787613
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG17400.22092068058805528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATGA301.22709025E-895.047931
TTAGGGA301.22709025E-895.047931
TGGAGGA156.603894E-495.047931
TCTTGGA156.603894E-495.047931
GTTCTTA156.603894E-495.047931
ATGATGA156.603894E-495.047931
GGTCGTA156.603894E-495.047931
GTTTCCA156.603894E-495.047931
CGGTTGA156.603894E-495.047931
CGTTGCA156.603894E-495.047931
TCGGGGA301.22709025E-895.047931
AATGGGA301.22709025E-895.047931
TGCGGTA156.603894E-495.047931
CGTTCGA156.603894E-495.047931
TGTTGGA301.22709025E-895.047931
CGGGGGA156.603894E-495.047931
GCATTTA301.22709025E-895.047931
GTTGTGA156.603894E-495.047931
GGGCGTA301.22709025E-895.047931
CCTTTGA301.22709025E-895.047931
Produced by FastQC (version 0.11.4)