FastQCFastQC Report
Tue 11 Oct 2022
A734_Diaphragm_S16_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA734_Diaphragm_S16_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998390
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20850.20883622632438226No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13800.1382225382866415No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTTG201.7534352E-594.893721
ATCCTGG201.7534352E-594.893721
GGAATGG600.094.893721
CAGCGGA201.7534352E-594.893722
AGGTACG254.6511195E-794.893721
GACAGGA156.647178E-494.893722
AGCAACG156.647178E-494.893721
GGCAGAC500.094.893723
GAAGCGA201.7534352E-594.893722
ACACGGA301.2414603E-894.893722
GAGCTGG201.7534352E-594.893721
GTACAGT750.094.893722
AGATACG201.7534352E-594.893721
CAACGCG156.647178E-494.893721
ACGCATG254.6511195E-794.893721
TAGACGA254.6511195E-794.893722
GACACGG201.7534352E-594.893721
TGGACGT550.094.893722
CAGCAGG156.647178E-494.893721
GACCGAA550.094.893723
Produced by FastQC (version 0.11.4)