FastQCFastQC Report
Tue 11 Oct 2022
A734_Diaphragm_S16_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA734_Diaphragm_S16_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998390
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG14210.14232914993138954No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA156.608433E-495.033391
TTGATGA600.095.033391
CGGGTTA254.610556E-795.033391
TTTAGGA500.095.033391
ATGGGGA254.610556E-795.033391
CGTGGCA201.7406775E-595.033391
GGCAGGA156.608433E-495.033391
TCGTTGA301.2287273E-895.033391
GCCTTGA156.608433E-495.033391
TCCGCGA201.7406775E-595.033391
GGTGTCA301.2287273E-895.033391
TTGTGGA409.094947E-1295.033391
TTGTGCA156.608433E-495.033391
ATAGGGA409.094947E-1295.033391
TGCTTCA156.608433E-495.033391
CGCGGTA156.608433E-495.033391
GGATGTA500.095.033391
GTTTCGA201.7406775E-595.033391
GGATGGA450.095.033391
GCGGTGA156.608433E-495.033391
Produced by FastQC (version 0.11.4)