FastQCFastQC Report
Tue 11 Oct 2022
A732_SpCord_S42_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA732_SpCord_S42_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554162
Sequences flagged as poor quality0
Sequence length101
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT1250.094.8932656
TCACGGA156.6457316E-494.8932653
TCCGGCC950.094.8932654
TCTGCGG156.6457316E-494.8932651
AACGTGC301.2403689E-894.8932652
GGCAGTG156.6457316E-494.8932651
CGGCGGC156.6457316E-494.8932653
GCCCATC2550.094.8932657
GCCCAAT2400.094.8932657
GAAGCAC1250.094.8932654
TGCCACC156.6457316E-494.8932657
TCATTGC600.094.8932652
TTAGCAC5000.094.8932654
TCCGAGC156.6457316E-494.8932652
TCCTGCC301.2403689E-894.8932653
AGCCCCA156.6457316E-494.8932655
GGCAATA156.6457316E-494.8932651
GATCCTG156.6457316E-494.8932651
GACCGCA156.6457316E-494.8932653
GCGCCAT156.6457316E-494.8932656
Produced by FastQC (version 0.11.4)