FastQCFastQC Report
Tue 11 Oct 2022
A732_SpCord_S42_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA732_SpCord_S42_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554162
Sequences flagged as poor quality0
Sequence length101
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG8780.1584374244354539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA254.6052628E-795.0422741
GTTTCGA254.6052628E-795.0422741
TTTCGGA254.6052628E-795.0422741
GTAGGTA254.6052628E-795.0422741
TATGCGA254.6052628E-795.0422741
ACGATCA254.6052628E-795.0422741
GATGGTA254.6052628E-795.0422741
GGGCGTA254.6052628E-795.0422741
CGTACTA254.6052628E-795.0422741
AGCGAAA254.6052628E-795.0422741
GCATGGA254.6052628E-795.0422741
CGTTTCA254.6052628E-795.0422741
AGTACGA254.6052628E-795.0422741
TTGGGGA254.6052628E-795.0422741
GGGATGA254.6052628E-795.0422741
GTACGAA254.6052628E-795.0422741
ACGGTTA254.6052628E-795.0422741
GTGTTTA550.095.042271
GTTTGGA301.22690835E-895.042271
TTTAGCA201.739183E-595.042271
Produced by FastQC (version 0.11.4)