FastQCFastQC Report
Tue 11 Oct 2022
A732_Liver_S36_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA732_Liver_S36_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788146
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATG450.094.9103551
AAGGTGC450.094.9103552
AATGAGC450.094.9103552
AGGCTTG1050.094.9103551
ATCCTGC409.094947E-1294.910352
GATACTG409.094947E-1294.910351
GTACCTG254.6477726E-794.910351
AGTCCCG201.7522916E-594.910351
GATCCTG254.6477726E-794.910351
GGGAGTC201.7522916E-594.910353
AAGCAGC254.6477726E-794.910352
GTACTGC750.094.910352
AGCGAGC254.6477726E-794.910352
GCAATGC850.094.910352
GCTACGC254.6477726E-794.910352
GACCTGC254.6477726E-794.910352
AGCAGTG650.094.910351
GCTCCGC254.6477726E-794.910352
AGGTCCG201.7522916E-594.910351
AATCCGC156.643419E-494.910342
Produced by FastQC (version 0.11.4)