FastQCFastQC Report
Tue 11 Oct 2022
A732_Liver_S36_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA732_Liver_S36_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788146
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG39600.22145842677275793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA500.095.0160141
GATGGTA409.094947E-1295.0160141
GATGCGA450.095.0160141
AGGGGTA450.095.0160141
TGCAGGA254.6170862E-795.0160141
GCGGTGA156.6141016E-495.016011
GGATGCA301.2309101E-895.016011
GGTCCGA301.2309101E-895.016011
GTAGGTA950.095.016011
CGATTCA301.2309101E-895.016011
GCAGGGA156.6141016E-495.016011
GAGGGGA301.2309101E-895.016011
CGAAGGA301.2309101E-895.016011
GGTGCTA353.2923708E-1095.0161
TGAGGCA353.2923708E-1095.0161
TGTTTCG409.094947E-1294.992098
ATTGTGT301.2330929E-894.992087
AGTGGTT1700.094.992087
AGTGCGT650.094.992087
AGTTGCT17100.094.992087
Produced by FastQC (version 0.11.4)