FastQCFastQC Report
Tue 11 Oct 2022
A732_FrCx_S48_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA732_FrCx_S48_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403752
Sequences flagged as poor quality0
Sequence length101
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCAT2850.094.888186
GGGGCAA254.6476816E-794.8881764
TGATCCA156.6458294E-494.8881763
TCTGCAC2500.094.8881764
AGACTTG254.6476816E-794.8881761
GAGTGGA500.094.8881763
GACAGCC156.6458294E-494.8881763
GTCGCAC156.6458294E-494.8881764
TGCCCAC8950.094.8881766
GTTCTGC450.094.8881762
AATTTGC900.094.8881762
AGTCACA254.6476816E-794.8881765
GTCTGCA301.2400051E-894.8881763
GGATGGA254.6476816E-794.8881763
CGTGCAC1000.094.8881764
TCTTCTG353.3287506E-1094.8881761
TCACACC450.094.8881767
CGGCCAA750.094.8881766
GGCACAC6700.094.8881766
CAGTTGC156.6458294E-494.8881762
Produced by FastQC (version 0.11.4)