FastQCFastQC Report
Tue 11 Oct 2022
A732_FrCx_S48_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA732_FrCx_S48_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403752
Sequences flagged as poor quality0
Sequence length101
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG6140.15207355010006143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTA254.5990782E-795.055791
TTGATGA254.5990782E-795.055791
TTTAGCA254.5990782E-795.055791
AGTTTTA500.095.055791
TCATTCA254.5990782E-795.055791
TAGGTTA500.095.055791
AGGGTTA254.5990782E-795.055791
TGTATGA254.5990782E-795.055791
GAGGTTA254.5990782E-795.055791
AGGTTTA254.5990782E-795.055791
AGTTGTA254.5990782E-795.055791
GGTGGGA254.5990782E-795.055791
TGGGGGA254.5990782E-795.055791
AAGGGTA254.5990782E-795.055791
TTTTGCA254.5990782E-795.055791
ATTTGTA500.095.055791
TGTGTGA254.5990782E-795.055791
TGGCTTA254.5990782E-795.055791
GTTATGA500.095.055791
GGAGGAA254.5990782E-795.055791
Produced by FastQC (version 0.11.4)