FastQCFastQC Report
Tue 11 Oct 2022
A732_DRG_S54_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA732_DRG_S54_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466158
Sequences flagged as poor quality0
Sequence length101
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATGC900.094.889472
ATCCTGC301.2421879E-894.889472
TATCAGC500.094.889472
GGGGATA301.2421879E-894.889471
GGGGACT301.2421879E-894.889473
AATCCGC201.7541004E-594.889472
GCGACGC156.6489883E-494.889472
GCGACAT950.094.889476
TCTCTGC409.094947E-1294.889472
AGCCCGC254.6534478E-794.889472
ATGCTGC201.7541004E-594.889472
ATTCGAC301.2421879E-894.889474
CGCGAGC156.6489883E-494.889472
AGGCGAC254.6534478E-794.889474
ATGTCGC301.2421879E-894.889472
TCGGCCC1700.094.889474
CATTAGC700.094.889472
GCTCTGC301.2421879E-894.889472
TTCCCGC254.6534478E-794.889472
GAGACGC156.6489883E-494.889472
Produced by FastQC (version 0.11.4)