FastQCFastQC Report
Tue 11 Oct 2022
A732_DRG_S54_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA732_DRG_S54_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466158
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG24100.16437519012275623No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA156.6071405E-495.040321
CGTTGCA156.6071405E-495.040321
TAGCGAA301.2285454E-895.040321
AAGCGTA301.2285454E-895.040321
TGCGGGA600.095.040321
GTATGTA600.095.040321
CGTAGCA156.6071405E-495.040321
GTCCGTA156.6071405E-495.040321
TAGGGGA156.6071405E-495.040321
TGGGGCA156.6071405E-495.040321
GATGCGA156.6071405E-495.040321
CGATGGA301.2285454E-895.040321
CGATGCA156.6071405E-495.040321
AGGATGA301.2285454E-895.040321
GAGGGGA301.2285454E-895.040321
CTCAGTA156.6071405E-495.040321
TCCGCAA254.6096466E-795.040311
GTTTCCA254.6096466E-795.040311
CCGATGA353.2923708E-1095.040311
CGGTTTA750.095.040311
Produced by FastQC (version 0.11.4)