FastQCFastQC Report
Tue 11 Oct 2022
A731_SpCord_S43_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA731_SpCord_S43_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644929
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCC353.3287506E-1094.890923
ATTAAGC700.094.890922
TCGGCCC353.3287506E-1094.890924
ATCAAGC353.3287506E-1094.890922
GGCCATC2800.094.890927
GTCAGGA353.3287506E-1094.890923
CGAGCAC700.094.890924
AAGTGCC353.3287506E-1094.890923
TCAACTG353.3287506E-1094.890921
TCAGTGC353.3287506E-1094.890922
TCTGCCC1350.094.8909154
TCTGCAC3200.094.8909154
TATCAGC156.6468836E-494.8909152
AGACTGC201.7532198E-594.8909152
GGGGACT156.6468836E-494.8909153
GTACCGC156.6468836E-494.8909152
GGAGCAC650.094.8909154
GATGTGC550.094.8909152
CATATGC650.094.8909152
ATCTAGC450.094.8909152
Produced by FastQC (version 0.11.4)