FastQCFastQC Report
Tue 11 Oct 2022
A731_SpCord_S43_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA731_SpCord_S43_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644929
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG11180.17335241553721417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATGA353.2923708E-1095.04891
AGCGTTA201.738798E-595.04891
TCGTTGA353.2923708E-1095.04891
GATGTTA353.2923708E-1095.04891
AGTTTTA650.095.04891
GAGCTTA156.603075E-495.04891
GATGTCA409.094947E-1295.04891
TTGTGTA301.22672645E-895.04891
ATAGGGA156.603075E-495.04891
GGTCGCA201.738798E-595.04891
GTTTCGA156.603075E-495.04891
GGATGGA156.603075E-495.04891
ACGTTCA156.603075E-495.04891
CCGATAA201.738798E-595.04891
GCAACGA201.738798E-595.04891
TTTCGGA254.6042078E-795.04891
GGCGTTA254.6042078E-795.04891
TTTCGCA156.603075E-495.04891
TAGCGTA156.603075E-495.04891
ATGTGGA254.6042078E-795.04891
Produced by FastQC (version 0.11.4)