FastQCFastQC Report
Tue 11 Oct 2022
A731_Liver_S37_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA731_Liver_S37_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123555
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATGC550.094.897832
AGCAATG500.094.897831
AGACTGC156.6462543E-494.897832
AGGTAGC156.6462543E-494.897832
AACCGCC156.6462543E-494.897833
AATCATG600.094.897831
GCGGTGC254.650301E-794.897832
GGATGCA301.2412784E-894.897833
AGCCCGG156.6462543E-494.897832
CGGCCAT2650.094.897836
GGACTGC156.6462543E-494.897832
GATCCCG254.650301E-794.897831
GTGATAC254.650301E-794.897832
ATAGAGC254.650301E-794.897832
CGACATT600.094.897837
GCGTTGC500.094.897832
ATCACGC254.650301E-794.897832
GTAATGC1500.094.897832
AACGGTC156.6462543E-494.897833
ACGATGC254.650301E-794.897832
Produced by FastQC (version 0.11.4)