FastQCFastQC Report
Tue 11 Oct 2022
A731_Liver_S37_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA731_Liver_S37_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123555
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG21380.1902888599133999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTA500.095.0294951
GTAGCGA254.6120658E-795.0294951
AGCGATA254.6120658E-795.0294951
GGCGCAA254.6120658E-795.0294951
GAAGCGA156.6097284E-495.029491
GAGCTTA450.095.029491
TGTGGCA301.229273E-895.029491
AGGGGTA550.095.029491
GGCGCTA201.7411274E-595.029491
AGAGGGA409.094947E-1295.029491
GAAGGTA409.094947E-1295.029491
AGTTTCT5400.094.999877
AGTTGCT11000.094.999877
GTTCTAC201.7453676E-594.9829562
AAGGTAC201.7453676E-594.9829562
AGGCAAC201.7453676E-594.9829562
TGGGGAC201.7453676E-594.9829562
AAGGCAT201.7453676E-594.9829562
TAAGCAC201.7453676E-594.9829562
AGGTAAC301.2334567E-894.982952
Produced by FastQC (version 0.11.4)