FastQCFastQC Report
Tue 11 Oct 2022
A731_FrCx_S49_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA731_FrCx_S49_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739750
Sequences flagged as poor quality0
Sequence length101
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAC201.7534101E-594.890712
AACGTGC353.3287506E-1094.890712
GACAGCG8150.094.890718
GAAGCAC2200.094.890714
GGAGCCC450.094.890714
GGAGCAC409.094947E-1294.890714
TGCCACC201.7534101E-594.890717
GGATGGA353.3287506E-1094.890713
TCACACC201.7534101E-594.890717
GCAACCG254.650792E-794.890711
ATTACGC254.650792E-794.890712
ATAGCAC3700.094.890714
GATTTGC1150.094.890712
TTACTGC450.094.890712
AAGGTCA201.7534101E-594.890715
ATCACTG409.094947E-1294.890711
TGCGGCA450.094.890713
TATGCAC9900.094.890714
GGCCCAC7250.094.890716
AGTATGC750.094.890712
Produced by FastQC (version 0.11.4)