FastQCFastQC Report
Tue 11 Oct 2022
A731_FrCx_S49_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA731_FrCx_S49_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739750
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG11780.1592429874957756No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA156.606767E-495.036941
AATCCGA156.606767E-495.036941
GCAAGTA156.606767E-495.036941
AGTTTGA550.095.036941
GGTCGTA201.7400544E-595.036941
ACGTTCA201.7400544E-595.036941
TCTTCGA201.7400544E-595.036941
TAGGTTA409.094947E-1295.036941
TAGGTGA301.22799975E-895.036941
ATGTGGA301.22799975E-895.036941
TAGCGGA156.606767E-495.036941
GGTCCGA156.606767E-495.036941
AGGGTCA201.7400544E-595.036941
TCGATCA156.606767E-495.036941
ATCGTCA156.606767E-495.036941
AATGCGA301.22799975E-895.036941
TCTGTTA450.095.036941
GTCCGTA156.606767E-495.036941
CGAATTA156.606767E-495.036941
TAGGGGA201.7400544E-595.036941
Produced by FastQC (version 0.11.4)