FastQCFastQC Report
Tue 11 Oct 2022
A731_DRG_S55_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA731_DRG_S55_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398776
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGC450.094.8913042
ATCCTGC409.094947E-1294.89132
AAGTAGC500.094.89132
GCCTTGC950.094.89132
GGATGCA1250.094.89133
CGACGAC156.649E-494.89134
GAGTCGC156.649E-494.89132
CGCTGCG201.7541617E-594.89131
GCGTTGC1150.094.89132
ACGATGC750.094.89132
GAGACGC201.7541617E-594.89132
TGCTGCG156.649E-494.89131
AGCTCTG201.7541617E-594.89131
GGCGCAC2300.094.89134
GGCGATA201.7541617E-594.89131
TGGTCAT201.7541617E-594.89136
AGTAACG254.6538298E-794.89131
GAGGCCT301.2423698E-894.89135
GATGCGC353.3287506E-1094.891292
GGACATT37300.094.76417
Produced by FastQC (version 0.11.4)