FastQCFastQC Report
Tue 11 Oct 2022
A731_DRG_S55_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA731_DRG_S55_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398776
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG36730.15311975774311565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCA254.609774E-795.042961
ATGCGCA254.609774E-795.042961
TCCGCTA156.6069275E-495.042951
TAGGGTA600.095.042951
AGCGTAC450.094.995392
AACCGAT201.7446388E-594.995392
TCGTTAC550.094.995392
TAGGTAC500.094.995392
TAGCGAT201.7446388E-594.995392
AGGCAAC201.7446388E-594.995392
AAGGGAC500.094.995392
AGGGGAT353.3105607E-1094.995392
TTCGCAT254.6235436E-794.995392
ATTGAAC1250.094.995392
TTCGAAC550.094.995392
AGGGAAC550.094.995392
GAAGGAT353.3105607E-1094.995392
TAGTTAT750.094.9953842
TTTGCAC156.620088E-494.9953842
ACCCGAT156.620088E-494.9953842
Produced by FastQC (version 0.11.4)