FastQCFastQC Report
Tue 11 Oct 2022
A730_TA_S6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA730_TA_S6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117058
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19090.1708953339934005No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15770.14117440634237435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATG550.094.890341
GGCAGAC254.6524656E-794.890343
ACACGTC353.3287506E-1094.890343
CGGCCGT254.6524656E-794.890342
AGCCCAT254.6524656E-794.890346
AGAAGAC450.094.890343
AGGCGTC650.094.890343
GCTGACG254.6524656E-794.890341
TGCTGAC254.6524656E-794.890343
GATGCGA353.3287506E-1094.890342
GCCTAGG254.6524656E-794.890341
GGCATGA450.094.890342
TTCGGCG254.6524656E-794.890341
GCGTGAC254.6524656E-794.890343
AGGATGA700.094.890342
CGCAATC650.094.890345
AAGGCGT500.094.890342
GATTAGG550.094.890341
AGCAGTC550.094.890343
GCCCGAC254.6524656E-794.890343
Produced by FastQC (version 0.11.4)