FastQCFastQC Report
Tue 11 Oct 2022
A730_TA_S6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA730_TA_S6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117058
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG24850.22245935305060258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTA700.095.039071
ATGGGGA254.6092828E-795.039071
TTAGGGA700.095.039071
GCCTTCA201.7402519E-595.039071
CTCGTGA201.7402519E-595.039071
GGGCTTA254.6092828E-795.039071
ATAGGTA353.2923708E-1095.039071
GGTCGGA254.6092828E-795.039071
ATAGGGA550.095.039071
TCTTCCA254.6092828E-795.039071
TAGGTTA500.095.039071
TGGGTTA500.095.039071
GGCGTTA409.094947E-1295.039071
CGTTGTA254.6092828E-795.039071
TATGGGA409.094947E-1295.039071
CCGTGAA201.7402519E-595.039071
GCGCCTA201.7402519E-595.039071
AAGGTTA700.095.039071
GTGATGA500.095.039071
TTCCGTA254.6092828E-795.039071
Produced by FastQC (version 0.11.4)