FastQCFastQC Report
Tue 11 Oct 2022
A730_Liver_S18_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA730_Liver_S18_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3443650
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94700.274998911039159No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58040.16854209922611182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCC750.094.891916
GCCTGAC301.2425517E-894.8905263
ACAGCGC156.649464E-494.8905269
CAAGCGT650.094.8905262
AACCTCC301.2425517E-894.8905269
AGGCTAT353.3287506E-1094.8905264
GATCCGT900.094.890522
GCTGACT1800.094.890524
ACGCTAT201.7543418E-594.890524
CAGCCGG301.2427336E-894.889151
CATGATG750.094.889151
ATCCATG600.094.889151
CCCTTCG156.6498475E-494.889151
GAGCCCG156.6498475E-494.889151
GCTCGCG156.6498475E-494.889151
GCTCCCG156.6498475E-494.889151
ATCCTGG409.094947E-1294.8891451
CATTGCG201.7544684E-594.8891451
ATCAGCG353.3287506E-1094.8891451
ATGCTGG550.094.8891451
Produced by FastQC (version 0.11.4)