FastQCFastQC Report
Tue 11 Oct 2022
A730_Liver_S18_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA730_Liver_S18_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3443650
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG131730.3825301642152948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCCA156.608737E-495.03731
GCAGGTA650.095.03731
ATTGGTT156.6167576E-495.008317
AACCGAT201.7445758E-594.997282
TTAGCAT409.094947E-1294.997282
GGCGTAC409.094947E-1294.997282
AGGCGAT409.094947E-1294.997282
GTAGCAT201.7445758E-594.997282
GTAGCAC409.094947E-1294.997282
GAGGTAT409.094947E-1294.997282
GGCGGAC409.094947E-1294.997282
GTTGCAC409.094947E-1294.997282
GTTCTAC500.094.997272
ATGGCAT301.232911E-894.997272
GCGGTAC254.6234345E-794.997272
GTAGGAT900.094.997272
GATGCAT301.232911E-894.997272
GATGCAC500.094.997272
GGTGCAT254.6234345E-794.997272
TTTGCAC1100.094.997272
Produced by FastQC (version 0.11.4)