FastQCFastQC Report
Tue 11 Oct 2022
A730_Heart_S12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA730_Heart_S12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848100
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14320.16884801320598986No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12330.14538379908029714No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAA353.3287506E-1094.888173
GCCCATC353.3287506E-1094.888177
ACACGTC353.3287506E-1094.888173
GCAAGGA353.3287506E-1094.888172
GGAGACG156.648378E-494.888171
ACGCATG301.2416422E-894.888171
GACACCA156.648378E-494.888177
TTCGTGA156.648378E-494.888172
CACGATG156.648378E-494.888171
TGACGAC301.2416422E-894.888173
GCTGACT850.094.888174
ATCCGAA353.3287506E-1094.888173
AGGCCCG353.3287506E-1094.888171
ACGGCCC156.648378E-494.888174
AACATGA353.3287506E-1094.888172
AATGAAG301.2416422E-894.888171
CGTAGAC301.2416422E-894.888173
GAGCGGA156.648378E-494.888172
ACAATGG353.3287506E-1094.888171
ACAGCCC156.648378E-494.888174
Produced by FastQC (version 0.11.4)