FastQCFastQC Report
Tue 11 Oct 2022
A730_Heart_S12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA730_Heart_S12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848100
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG17510.2064615021813465No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCA201.73848E-595.05591
ATGGGGA409.094947E-1295.05591
TCGTTGA409.094947E-1295.05591
CCGGGTA201.73848E-595.05591
AGTTTTA1150.095.05591
TTTGTCA201.73848E-595.05591
TTTCGTA201.73848E-595.05591
TATGGCA201.73848E-595.05591
TTATGCA201.73848E-595.05591
GGATCTA201.73848E-595.05591
TCGGGGA201.73848E-595.05591
TTCGTGA201.73848E-595.05591
GCGTTTA409.094947E-1295.05591
ACGGGTA201.73848E-595.05591
TCGTGGA201.73848E-595.05591
ATTTTTA1250.095.05591
GCTTCGA201.73848E-595.05591
ATTTTGA800.095.05591
GTTGTGA201.73848E-595.05591
TTCTTGA201.73848E-595.05591
Produced by FastQC (version 0.11.4)