FastQCFastQC Report
Tue 11 Oct 2022
A730_Gastroc_S9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA730_Gastroc_S9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790394
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13190.166878797156861No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10670.13499596403818856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGTC900.094.90013
GTTTGAC600.094.900093
TTAGGTC550.094.900093
AGCCGTC156.6448876E-494.900093
GGCAGTC650.094.900093
AGCAACG201.7526234E-594.900091
CGGCGAA156.6448876E-494.900093
GATACGG353.3287506E-1094.900091
GCCTTGA201.7526234E-594.900092
ACACGAC156.6448876E-494.900093
GAGCTTG156.6448876E-494.900091
TACTTCG156.6448876E-494.900091
GTACACG156.6448876E-494.900091
CAACGTC409.094947E-1294.900093
CAACGAA550.094.900093
AGGAGAC156.6448876E-494.900093
GGATGCG201.7526234E-594.900091
CAGCCGT201.7526234E-594.900092
TGGACGT409.094947E-1294.900092
GCAACGA156.6448876E-494.900092
Produced by FastQC (version 0.11.4)