FastQCFastQC Report
Tue 11 Oct 2022
A730_Gastroc_S9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA730_Gastroc_S9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790394
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG15950.20179809057254988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA301.2283635E-895.033621
GTGTTCA254.609592E-795.033621
TGTTTGA301.2283635E-895.033621
TTTAGGA600.095.033621
GTTTGCA201.7404305E-595.033621
CGGGTCA156.6078536E-495.033621
CGTGGGA156.6078536E-495.033621
TGGAGGA254.609592E-795.033621
GGCAGGA156.6078536E-495.033621
TTGGCCA156.6078536E-495.033621
TCTTGGA254.609592E-795.033621
AGATCTA353.2923708E-1095.033621
GATGTGA550.095.033621
GAGCTTA301.2283635E-895.033621
TTGTGGA353.2923708E-1095.033621
ATAGGGA201.7404305E-595.033621
GGATGTA301.2283635E-895.033621
GGATGGA156.6078536E-495.033621
GCGGTAA201.7404305E-595.033621
TTGCTGA254.609592E-795.033621
Produced by FastQC (version 0.11.4)