FastQCFastQC Report
Tue 11 Oct 2022
A730_Diaphragm_S15_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA730_Diaphragm_S15_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009524
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18250.18077826777768533No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13620.13491506888394927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATC32500.094.887978
ATGGAGG750.094.887971
AGGACTC5600.094.887975
TGCGGGA700.094.887972
GACCCGT353.3287506E-1094.887972
CAAGGAC353.3287506E-1094.887973
ATGGTGA750.094.887972
AGGTGAC353.3287506E-1094.887973
CGAAGTC353.3287506E-1094.887973
GACAGTG254.6528294E-794.887961
GAGGAGG201.7539734E-594.887961
AATCCTG201.7539734E-594.887961
GTACCTG156.6488027E-494.887961
AGCCGAC201.7539734E-594.887963
ACAAACG156.6488027E-494.887961
GAAGCAC156.6488027E-494.887963
ACACGGA201.7539734E-594.887962
ACACGCG201.7539734E-594.887961
ATTAGAC409.094947E-1294.887963
GAGCTGG254.6528294E-794.887961
Produced by FastQC (version 0.11.4)