FastQCFastQC Report
Tue 11 Oct 2022
A730_Diaphragm_S15_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA730_Diaphragm_S15_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009524
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG18520.18345279557494423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGA650.095.040161
TTTAGGA650.095.040161
TAGGGTA650.095.040161
TGGATGA650.095.040161
TTTTGGA650.095.040161
TGATCGA201.7400718E-595.040151
GTTTGTA450.095.040151
AACGTTA201.7400718E-595.040151
TGTTTGA800.095.040151
TTAGGTA700.095.040151
CGTGGCA201.7400718E-595.040151
TGGAGCA156.606583E-495.040151
TCGTTGA353.2923708E-1095.040151
GGAGCGA156.606583E-495.040151
AGTTTGA450.095.040151
ATTAGGA550.095.040151
TTGTGGA450.095.040151
GCTGGTA254.608646E-795.040151
GGATGCA254.608646E-795.040151
GGGTCTA254.608646E-795.040151
Produced by FastQC (version 0.11.4)