FastQCFastQC Report
Tue 11 Oct 2022
A729_TA_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA729_TA_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257685
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20290.16132815450609653No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19180.15250241515164767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAT650.094.887896
GGCACAA2700.094.887896
CGTTGAA1300.094.887893
GCAGGAA1300.094.887893
GGACATC353.3287506E-1094.887897
ATGCCGA353.3287506E-1094.887892
ATCCTCC70150.094.8878869
GGCAGTC800.094.8878863
GAGGACA409.094947E-1294.8878865
GAGTGCG301.2421879E-894.8878861
AGCAACG301.2421879E-894.8878861
CCGGGAG201.7541488E-594.8878861
ACACGGC1600.094.8878869
TGCCCAC1650.094.8878866
ATAGGCC450.094.8878864
CGAAAGG409.094947E-1294.8878861
GAATGCG254.6535206E-794.8878861
GCAACGG201.7541488E-594.8878861
GCAAACG156.6492095E-494.8878861
GCACGAC254.6535206E-794.8878863
Produced by FastQC (version 0.11.4)