FastQCFastQC Report
Tue 11 Oct 2022
A729_TA_S5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA729_TA_S5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257685
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTA409.094947E-1295.0370941
GGTCGTA409.094947E-1295.0370941
TGAAGCA201.7405175E-595.0370941
TAGGTGA409.094947E-1295.0370941
TATGGGA750.095.0370941
GTAGGTA201.7405175E-595.0370941
TTCCGGA201.7405175E-595.0370941
AGGCGTA409.094947E-1295.0370941
CGCTGGA201.7405175E-595.0370941
GCGTTGA201.7405175E-595.0370941
GCCATGA201.7405175E-595.0370941
TTTGGGA750.095.0370941
TTCGGGA201.7405175E-595.0370941
TGTAGCA409.094947E-1295.0370941
TGAGGCA201.7405175E-595.0370941
CGCGTGA201.7405175E-595.0370941
GTGCGTA201.7405175E-595.0370941
GGAGGCA201.7405175E-595.0370941
TGGAGCA156.6078117E-495.037091
TCCATGA254.6101923E-795.037091
Produced by FastQC (version 0.11.4)