FastQCFastQC Report
Tue 11 Oct 2022
A729_Liver_S17_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA729_Liver_S17_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7880655
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG251820.3195419670065496No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127040.16120487446792175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT5250.094.890144
GCCTGAC650.094.890133
CAGGCCC156.6498935E-494.890134
AGCCCTG800.094.8895341
GATCCGT2500.094.8895342
GCCTTCG500.094.889531
GCAGGCT156.650061E-494.889532
ATCAACC973550.094.8401959
ATCATCC505750.094.8045049
ATCCTCC455250.094.7951359
ATCCACC503950.094.775969
AGGTATC222000.094.740535
ATCTACC468150.094.7166449
GTATCCT290300.094.6940167
AATCATC214050.094.690048
AGGAATC162600.094.685885
GAATCCT153000.094.673067
GGTATCC149500.094.6362466
AATCCTC158500.094.6201258
GGAATCC116900.094.606036
Produced by FastQC (version 0.11.4)