FastQCFastQC Report
Tue 11 Oct 2022
A729_Liver_S17_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA729_Liver_S17_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7880655
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG226100.2869050859351158No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCAT254.624817E-794.994382
AGGGCAT600.094.994382
GATGCAT900.094.994372
GCGCATT500.094.95523
AGCGATT1500.094.95523
TAGGACT3950.094.95523
TTAAACC3350.094.9546055
GCAATGT850.094.9546055
AAGTAGC8000.094.95466
GGTACTT1250.094.95464
TGATTGC3750.094.95465
ATAGTTT3200.094.95465
AAGTGGC591800.094.8984456
TAAGTGG216750.094.8888855
GCAGTGG79750.094.775995
AAAGTGG202600.094.767125
AAGACGC24800.094.763166
AGTGGCT1813550.094.754777
AGTTGCT92350.094.7516567
TTTAGTG133650.094.705934
Produced by FastQC (version 0.11.4)