FastQCFastQC Report
Tue 11 Oct 2022
A729_Heart_S11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA729_Heart_S11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704519
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11610.16479328449623076No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9040.12831449542169907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCC39850.094.889729
AGCACAC1350.094.889716
ATGGGCA156.6474767E-494.889713
AACAAGG353.3287506E-1094.889711
ACACGGA156.6474767E-494.889712
TGCCCAA1200.094.889716
TGCCACA156.6474767E-494.889715
AGATACG254.650865E-794.889711
TGAAGGC156.6474767E-494.889713
AGGAGAC450.094.889713
ACGCATG156.6474767E-494.889711
AACCCTG156.6474767E-494.889711
GACAATC900.094.889715
TAGCGTG156.6474767E-494.889713
GGACTGT254.650865E-794.889712
TGGCGAC156.6474767E-494.889713
TTGTCCG156.6474767E-494.889711
AGGACGA353.3287506E-1094.889712
GAATCCA25800.094.889717
GCGCCCA156.6474767E-494.889715
Produced by FastQC (version 0.11.4)