FastQCFastQC Report
Tue 11 Oct 2022
A729_Heart_S11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA729_Heart_S11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704519
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCA201.7392407E-595.045261
TTGATGA301.22727215E-895.045261
TTTAGGA450.095.045261
ATGGGGA409.094947E-1295.045261
CTATGGA201.7392407E-595.045261
CGTGGGA156.6043384E-495.045261
CGTGGCA156.6043384E-495.045261
GTTCTTA409.094947E-1295.045261
CTCGTTA254.6057175E-795.045261
GATGTGA254.6057175E-795.045261
GAGCTTA301.22727215E-895.045261
AGTTTGA409.094947E-1295.045261
ATTAGGA301.22727215E-895.045261
GGTGTCA254.6057175E-795.045261
ATAGGTA201.7392407E-595.045261
TTGTGGA254.6057175E-795.045261
GGATGGA201.7392407E-595.045261
GGATGAA201.7392407E-595.045261
CCAGGTA301.22727215E-895.045261
TTTCGTA409.094947E-1295.045261
Produced by FastQC (version 0.11.4)