FastQCFastQC Report
Tue 11 Oct 2022
A729_Gastroc_S8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA729_Gastroc_S8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171385
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19090.1629694763036918No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16390.1399198384817972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGAC301.2418241E-894.891243
GCCCAAC600.094.891247
AGGACTC5300.094.891245
ACGGGCC301.2418241E-894.891244
GGCCCAC1100.094.891246
CCCAACG600.094.891248
GTTAAGC156.64816E-494.891242
TGCGACA156.64816E-494.891245
ACAGCGG156.64816E-494.891241
AGCTGCG156.64816E-494.891241
GCGATGA301.2418241E-894.891242
GATGCCC301.2418241E-894.891244
AGGGCCC301.2418241E-894.891244
ACGAGAC301.2418241E-894.891243
TGAGCAC301.2418241E-894.891244
CCTGCCC301.2418241E-894.891244
GACTACG156.64816E-494.891241
GCTCATG156.64816E-494.891241
TGCCGCG156.64816E-494.891241
GCGAGAC254.6523564E-794.8912353
Produced by FastQC (version 0.11.4)